Geometric Alignment of 2D Gel Electrophoresis Images

Geometric Alignment of 2D Gel Electrophoresis Images

OBJECTIVES 2D gel electrophoresis (2-DE) is the method of choice for analyzing protein expression in the field of proteomics, for example, comparing a reference with a test population. However, due to complex physical and chemical processes the locations of proteins generally vary in different 2-DE images. To cope with these variations, accurate geometric alignment of 2-DE images is important. ...

Segmentation of 2d-gel Electrophoresis Images

Introduction Two-Dimensional Gel Electrophoresis technique is a convenient and well-established method to separate thousands of proteins on polyacrylamide gels, according to the differences in their net charge and their molecular mass [1]. Its digital output is an image which depicts proteins as bright or dark spots over a noisy and inhomogeneous background. Each protein is characterized by its...

2D Gel Electrophoresis - An Overview

Automated image alignment for 2D gel electrophoresis in a high-throughput proteomics pipeline

MOTIVATION The quest for high-throughput proteomics has revealed a number of challenges in recent years. Whilst substantial improvements in automated protein separation with liquid chromatography and mass spectrometry (LC/MS), aka 'shotgun' proteomics, have been achieved, large-scale open initiatives such as the Human Proteome Organization (HUPO) Brain Proteome Project have shown that maximal p...

Warping two-dimensional electrophoresis gel images to correct for geometric distortions of the spot pattern.

A crucial step in two-dimensional gel based protein expression analysis is to match spots in different gel images that correspond to the same protein. It still requires extensive and time-consuming manual interference, although several semiautomatic techniques exist. Geometric distortion of the protein patterns inherent to the electrophoresis procedure is one of the main causes of these difficu...